About - eLife
eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science.
Latest research | eLife
Oct 4, 2024 · eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science. eLife Sciences Publications, Ltd is a limited liability non-profit non-stock corporation incorporated in the State of Delaware ...
Endoplasmic Reticulum: Keeping in shape - eLife
Sep 13, 2016 · The endoplasmic reticulum is the largest single structure in eukaryotic cells. It consists of a range of interconnected shapes, including sheets and tubules, and comprises a lumen enclosed by a membrane that is continuous with the membrane that surrounds the nucleus of the cell ().The structure and dynamic nature of the endoplasmic reticulum allow it to be involved in many processes in cells ...
How to measure and evaluate binding affinities | eLife
Aug 6, 2020 · In the first, ‘binding’ regime, the concentration of the constant (‘trace’) component, R, is well below the dissociation constant ( [R] total << KD for the example in Figure 3). In this case, the concentration of the variable component (P in Figure 3) that gives half binding is equal to the KD (Figure 5A).
Magazine - eLife
eLife is a non-profit organisation inspired by research funders and led by scientists. Our mission is to help scientists accelerate discovery by operating a platform for research communication that encourages and recognises the most responsible behaviours in science. eLife Sciences Publications, Ltd is a limited liability non-profit non-stock …
Publishing and peer review at eLife · eLife
2 Peer review. eLife editors - who are all active researchers - discuss new submissions and decide which will be peer reviewed. The criteria used by the editors are discussed in “How and why eLife selects papers for peer review”.. Each peer reviewer is asked to write a Public Review of the article, and to recommend ways in which the authors could improve it.
The Natural History of Model Organisms: The secret lives of
Jun 4, 2015 · D. melanogaster, described by Meigen in 1830, appears to have originated in sub-Saharan Africa (Lachaise et al., 1988).The first out-of-Africa habitat expansion of D. melanogaster is thought to have occurred between 10,000 and 15,000 years ago, when it moved to Europe and Asia (David and Capy, 1988).North America and Australia were colonized more recently (David and Capy, 1988).
Cell Division: How centrioles acquire the ability to reproduce
Mar 8, 2017 · Centrioles are organelles that have two critical functions. In dividing cells, they recruit a collection of proteins (known as pericentriolar material) to form larger organelles called centrosomes that nucleate microtubules and organize the spindle poles during cell division (Fu et al., 2015; Figure 1A) In non-dividing cells, centrioles are involved in …
Maximizing CRISPRi efficacy and accessibility with dual-sgRNA
Dec 28, 2022 · CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but wider deployment of CRISPRi screening has been constrained by the large size of single guide RNA (sgRNA ...
Ferredoxin 1 is essential for embryonic development and lipid ...
Jan 22, 2024 · This pipeline will support future drug target selection across diseases where therapeutic targeting of immune inhibitory receptors is warranted. The iron-sulfur cluster containing Ferredoxin 1 (FDX1), critical in steroidogenesis and TCA cycle, is required for mammalian embryonic development and maintenance of lipid homeostasis at cellular and ...